Composite

Part:BBa_K3783023:Experience

Designed by: Satvik Kethireddy   Group: iGEM21_OhioState   (2021-10-21)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3783023

OhioState Application

LAL Assay Graph
Figure 2. Endotoxin Units of Construct Lysates via LAL Assay

The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a noticeable decrease in bound endotoxin.

User Reviews

UNIQ763400fb17489b2d-partinfo-00000001-QINU UNIQ763400fb17489b2d-partinfo-00000002-QINU